Device

Part:BBa_M50497

Designed by: Matthew Prospero, Cami Tussie, Martin Alcaraz Jr.   Group: Stanford BIOE44 - S11   (2018-10-20)


uvrY Biosensor with lux Sp1 and luxSp2 Promoters

The sensor fluoresces based upon the presence of uvrY, with more fluorescence with increased uvrY. This device utilizes the luxSp1 and luxSp2 promoters in tandem to promote the production of GFP upon activation; these two promoters for the luxS gene can be induced through binding with UvrY, a known transcription regulator of the luxS gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 594
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 594
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 594
    Illegal BamHI site found at 803
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 594
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 594
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None